The first factory-installed Road King audio system includes a 200-watt amplifier and an interface module for an included 8GB Apple iPod controlled by buttons on the handlebar. The system drives two 5x7-inch speakers with bridged tweeters located on the saddlebag lids, and two 5.25-inch speakers with bridged tweeters mounted in the fairing lowers. The new detachable Vented Wind Splitter windshield was developed by Harley-Davidson and tested in the Wichita State University wind tunnel to relieve low pressure behind the windshield and significantly reduce buffeting at the rider position. A vane within the vent should be adjusted to match air flow to the rider's height. A low-profile console holds a combination speedometer/tachometer with programmable back-light colors.

Other CVO Road King features include a chrome billet-style extended reach heel shifter, Mirror Chrome Agitator Custom wheels, extended saddlebag bottoms with a custom fascia and LED lighting, a new chromed handlebar with an ergonomic bend, and Slipstream Collection hand and foot controls. A new solo touring seat and the removable passenger pillion and backrest have a tuck-and-roll leather insert. A new painting process features hand-finished graphics created with textures and solvents in a multi-step process that results in unique detailing on each motorcycle. Three color options are available, including the 110th anniversary scheme.

A highlight of each 110th Anniversary Edition model are solid bronze fuel tank badges, plated in black nickel and then distressed to highlight the bronze. A bright gold-tone Bar and Shield cloisonné is inserted in the main body of the single wing badge while each motorcycle will carry other model-specific anniversary badging, trim and a serialization badge.